Transcription by T7 RNA polymerase using benzo[a]pyrene-modified templates
نویسندگان
چکیده
منابع مشابه
Transcription of RNA templates by T7 RNA polymerase.
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was abl...
متن کاملProcessivity in early stages of transcription by T7 RNA polymerase.
Immediately following initiation of transcription, T7 RNA polymerase enters a phase in which dissociation of the enzyme-DNA-RNA ternary complex significantly competes with elongation, a process referred to in the Escherichia coli enzyme as abortive cycling [Carpousis, A.J., & Gralla, J.D. (1980) Biochemistry 19, 3245-3253]. Characterization of this process in the T7 RNA polymerase system under ...
متن کاملEffect of carcinogenic adducts on transcription by T7 RNA polymerase.
The effect of modifying the T7 promoter-containing plasmid pDR100 with aminofluorene (AF), acetylaminofluorene (AAF), and benzo[a]pyrene (B[a]P) adducts on RNA synthesis by the T7 RNA polymerase was determined. We found that increasing levels of each of the three adducts caused a progressive decline in RNA synthesis, but that the inhibition produced by benzo[a]pyrene adducts was substantially g...
متن کاملStructural Transitions Mediating Transcription Initiation by T7 RNA Polymerase
During transcription initiation, RNA polymerases appear to retain promoter interactions while transcribing short RNAs that are frequently released from the complex. Upon transition to elongation, the polymerase releases promoter and forms a stable elongation complex. Little is known about the changes in polymerase conformation or polymerase:DNA interactions that occur during this process. To ch...
متن کاملSynthetic polyamines stimulate in vitro transcription by T7 RNA polymerase.
The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short tr...
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ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1991
ISSN: 0143-3334,1460-2180
DOI: 10.1093/carcin/12.8.1531-a